The last packet successfully received from the server was 16 milliseconds ago.  The last packet sent successfully to the server was 16 milliseconds ago.

1. Ensure MySQL is running on the target server.
Windows
net start | findstr MySQL
Linux
systemctl status mysqld

2. Verify host and port configuration. 
SHOW VARIABLES LIKE 'port';
Ensure you are using this port while adding monitor

SHOW VARIABLES LIKE 'bind_address';
 Bind Address should be the Ip address of the Mysql Server 


3. To Monitor MySQL server version greater than 5.6 and later download the latest driver and place under the below location and restart the Applications Manager 
AppMangager Home/working/mysql/MMMySQLDriver