Disable users NOT in CSV file

Disable users NOT in CSV file

We import and create ad users from a CSV file built on these attributes
DEPARTMENT,GIVENNAME,USERPRINCIPALNAME,PWDLASTSET,HOMEDIRECTORY,DESCRIPTION

Once a user is removed from this CSV file, the corresponding active user account created has to be disabled. Right now we disable them using the "Last logon N days" value, but would like to run it daily and only disable users that no longer has a line in the file.

The CSV file is never edited, it's over written daily by a new file with the same name which contains an updated user list. 

Is this at all possible, or how should I go about solving this?